NOD2 Antibody | 2511

NOD2 Antibody | 2511 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: NOD2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2.
The immunogen is located within the first 50 amino acids of NOD2.

Research Area: Apoptosis

Tested Application: E, WB, ICC, IF

Application: NOD2 antibody can be used for detection of NOD2 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least four isoforms of NOD2 are known to exist; this antibody will only detect the longest isoform. NOD2 has no cross-reaction with NOD1.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: Cat. No. 1369 - Human Lymph node Tissue Lysate

Positive Control 3: Cat. No. 17-005 - Jurkat Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 114 kDa
Observed: 100 kDa

Validation: N/A

Isoform: N/A

Purification: NOD2 Antibody is immunoaffinity chromatography purified IgG.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NOD2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NOD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NOD2 Antibody: CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1, Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NOD2 Antibody: Apaf-1 and NOD1 are members of a new family, which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD) . A third member in this family was recently identified and designated NOD2. NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease that is a main type of chronic inflammatory bowel disease.