NOD3 Antibody | 5949

NOD3 Antibody | 5949 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: NOD3 antibody was raised against an 18 amino acid synthetic peptide near the center of human NOD3.
The immunogen is located within amino acids 630 - 680 of NOD3.

Research Area: Innate Immunity

Tested Application: E, WB, IF

Application: NOD3 antibody can be used for detection of NOD3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 2: Cat. No. 17-005 - Jurkat Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: NOD3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NOD3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NOD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NOD3 Antibody: NOD3, CLR16.2, NOD3, Protein NLRC3, CARD15-like protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NOD3 Antibody: NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3) , is predominantly expressed in the immune system, particularly in T lymphocytes, and its expression is strongly down-regulated following stimulation of the T-cell receptor complex and CD28, suggesting that NOD3 plays a role in attenuating the activation of T cells. NOD3 inhibits NF-kappaB, AP-1 and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or treatment with PMA/ionomycin and decreases IL-2 and CD25 mRNA induction in activated cells.