NOX1 Antibody | 7921

NOX1 Antibody | 7921 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (93%) , Mouse: (85%)

Immunogen: NOX1 antibody was raised against a 15 amino acid peptide near the center of human NOX1.
The immunogen is located within amino acids 210 - 260 of NOX1.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: NOX1 antibody can be used for detection of NOX1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: NOX1 antibody is human specific.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 62 kDa
Observed: 64 kDa

Validation: N/A

Isoform: N/A

Purification: NOX1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NOX1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NOX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: NOX1 Antibody: MOX1, NOH1, NOH-1, GP91-2, MOX1, NADPH oxidase 1, Mitogenic oxidase 1, NOX-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress (1) . NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox (1) . Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv (2) . NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H (+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain (1-3) . NOX1 have the potential to be effective treatments for a range of ischemic diseases (4) .