NOX2 Antibody | 7923

NOX2 Antibody | 7923 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (87%) , Bovine: (87%)

Immunogen: Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2.
The immunogen is located within amino acids 140 - 190 of NOX2.

Research Area: Immunology, Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 59, 63 kDa
Observed: 58 kDa

Validation: N/A

Isoform: N/A

Purification: NOX2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NOX2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NOX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: NOX2 Antibody: CGD, NOX2, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX, Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b558 subunit beta

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1) . NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2) . Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer’s and Parkinson’s disease (3) .