NPM1 Antibody [7H10B9] | 32-198

NPM1 Antibody [7H10B9] | 32-198 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Ni-NTA purified recombinant human NPM1 expressed in E. Coli strain BL21 (DE3) .

Research Area: Other

Tested Application: E, WB, IHC

Application: Western Blot:1:500 - 1:2, 000
IHC (P) :1:500 - 1:2, 000
IHC (F) :1:500 - 1:2, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Antibody is purified by protein A affinity chromatography.

Clonality: Monoclonal

Clone: 7H10B9

Isotype: IgG1

Conjugate: Unconjugated

Physical State: N/A

Buffer: N/A

Concentration: N/A

Storage Condition: NPM1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NPM, Nucleolar phosphoprotein B23, NPM, B23

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Nucleophosmin (NPM) , also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM1 has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities . The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase .