NPTX2 Antibody | 4573

NPTX2 Antibody | 4573 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)

Immunogen: Anti-NPTX2 antibody (4573) was raised against a peptide corresponding to 16 amino acids near the center of human NPTX2.
The immunogen is located within amino acids 170 - 220 of NPTX2.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: Cat. No. 1201 – HeLa Cell Lysate

Positive Control 3: Cat. No. 1211 – HepG2 Cell Lysate

Positive Control 4: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 47kD
Observed: 47 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar NPTX2 expression profile in human and mouse cell lines detected by two independent anti-NPTX2 antibodies that recognize different epitopes, 4573 against central domain and 64-234 against another central domain. NPTX2 proteins are detected only in HeLa and HepG2 cell lines by the two independent antibodies.

Isoform: Human NPTX2 has one isoform (431aa, 47kD) . Mouse NPTX2 has one isoform (429aa, 47.1kD) and Rat NPTX2 also has one isoform (432aa, 47.4kD) . 4573 can detect human, mouse and rat.

Purification: NPTX2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NPTX2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NPTX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NPTX2 Antibody: NP2, NARP, NP-II, Neuronal pentraxin-2, Neuronal pentraxin II, NP2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NPTX2 Antibody: Neuronal pentraxin (NPTX) 2 was initially identified as a member of the pentraxin family having high homology to NPTX1. Unlike NPTX1 however, NPTX2 is expressed in testes, pancreas, liver, heart, skeletal muscle as well as brain. NPTX2 possesses the lectin properties common to the pentraxin family, promotes neurite outgrowth, and is rapidly regulated by neuronal activity. NPTX1 and NPTX2 form heterocomplexes that contribute to both activity-independent and -dependent excitatory synaptogenesis. Recently, NPTX2 has been found to be highly upregulated in Parkinsonian substantia nigra and localizes to Lewy bodies and Lewy neurites in sporadic Parkinson's disease (PD) , suggesting that it is involved in the pathway dysregulation that underlies PD. This NPTX2 antibody is predicted to be specific to NPTX2 and not recognize NPTX1.