NSC-19630 | B2665

NSC-19630 (MIRA-1) is a cell-permeable inhibitor of Werner syndrome WRN helicase activity (IC₅₀ = 20 µM) over its ATPase and exonuclease activities, but did not affect other DNA helicases [Bloom syndrome (BLM), Fanconi anemia group J (FANCJ), RECQ1, RecQ, UvrD, or DnaB). Exposure of human cells to NSC 19630 dramatically impairs growth and proliferation, induces apoptosis in a WRN-dependent manner, and results in elevated γ-H2AX and proliferating cell nuclear antigen (PCNA) foci. NSC-19630 exposure leads to delayed S-phase progression, consistent with the accumulation of stalled replication forks, and to DNA damage in a WRN-dependent manner.

NSC-19630 (MIRA-1) is a cell-permeable inhibitor of Werner syndrome WRN helicase activity (IC₅₀ = 20 µM) over its ATPase and exonuclease activities, but did not affect other DNA helicases [Bloom syndrome (BLM), Fanconi anemia group J (FANCJ), RECQ1, RecQ, UvrD, or DnaB). Exposure of human cells to NSC 19630 dramatically impairs growth and proliferation, induces apoptosis in a WRN-dependent manner, and results in elevated γ-H2AX and proliferating cell nuclear antigen (PCNA) foci. NSC-19630 exposure leads to delayed S-phase progression, consistent with the accumulation of stalled replication forks, and to DNA damage in a WRN-dependent manner.

B2665 | NSC-19630 DataSheet

Alternate Name/Synonyms: 1-[(1-oxopropoxy)methyl]-1H-pyrrole-2,5-dione; MIRA-1

Appearance: White solid

Formulation:

CAS Number: 72835-26-8

Structure Available?:

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₈H₉NO₄

Molecular Weight: 183.16

Cell-Permeable?: True

Purity: ≥98% by HPLC

Solubilities: >15 mg/ml in DMSO, >15 mg/ml in Ethanol

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A WRN helicase inhibitor

MDL Number:

PubChem CID: 227681

SMILES: CCC(=O)OCN1C(=O)C=CC1=O

InChi: InChI=1S/C8H9NO4/c1-2-8(12)13-5-9-6(10)3-4-7(9)11/h3-4H,2,5H2,1H3

InChi Key: YXEWPGYLMHXLPS-UHFFFAOYSA-N