Assay also suitable for rat and mouse samples–NT-proANP as a cardiovascular safety biomarker in rats
NT-proANP ELISA Product Overview
The NT-proANP ELISA* is a 3.5 hour, 96-well sandwich ELISA for the quantitative determination of NT-proANP in human serum, EDTA plasma, heparın plasma, cell culture supernatants and urine.
*Please note that the name of this assay has been changed from proANP (1-98) ELISA to NT-proANP ELISA, referring to a more recent terminology for the peptide.
NT-proANP ELISA Assay Principle
This NT pro ANP ELISA kit is a sandwich enzyme immunoassay for the quantitative determination of NT proANP in human serum, EDTA plasma, heparın plasma, cell culture supernatants and urine. The NT-proANP ELISA has also been validated for rat samples.
The figure below explains the principle of a sandwich ELISA:
In a first step, sample and conjugate (sheep anti-human NT-proANP-HRP) are pipetted into the wells of the microtiter strips, which are pre-coated with polyclonal sheep anti-NT-proANP antibody. NT-proANP present in the sample binds to the pre-coated antibody in the well and forms a sandwich with the conjugate. In the washing step all non-specific unbound material is removed. In a second step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalyzed color change of the substrate is directly proportional to the amount of NT-proANP present in the sample. This color change is detectable with a standard microtiter plate ELISA reader.
The NT-proANP ELISA kit uses highly purified, epitope-mapped antibodies. The antibodies utilized in the NT pro ANP ELISA (BI-20892) are as follows:
Capture antibody: AA 1-30 (polyclonal sheep anti-human NT-proANP 1-98) Detection antibody: AA 79-98 (HRP-labeled polyclonal sheep anti-human NT-proANP 1-98)
NT-proANP ELISA Typical Standard Curve
The figure below shows a typical standard curve for the NT-proANP (or proANP (1-98)) ELISA. The immunoassay is calibrated against human recombinant NT-proANP (or proANP (1-98)) peptide:
NT-proANP ELISA Kit Components
Storage instructions: All reagents of the NT-proANP ELISA kit are stable at 4°C until the expiry date stated on the label of each reagent.
Rat N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP) ELISA Kit | DL-NT-ProANP-Ra | Gentaur UK, US & Europe Distribution Alternative Names: NT-Pro-ANP; N-ANP Species: Rattus...
Mouse N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP) ELISA Kit | DL-NT-ProANP-Mu | Gentaur UK, US & Europe Distribution Alternative Names: NT-Pro-ANP; N-ANP Species: Mus musculus...
Human N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP) ELISA Kit | DL-NT-ProANP-Hu | Gentaur UK, US & Europe Distribution Alternative Names: NT-Pro-ANP; N-ANP Species: Homo sapiens...