50

Nuclease P1 from Penicillium citrinum For pretreatment DNA samples to detect 8-OHdG

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Description

Nuclease P1 from Penicillium citrinum For pretreatment DNA samples to detect 8-OHdG

Description

Nuclease P1 is a zinc dependent glycoprotein that catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5'-phosphates and 5'-phospho-oligonucleotides.

Consists of 270 amino acid residues
Optimal pH: 5.3 (RNA and heat-denatured DNA); 7.2 (3'-AMP)
Stable below 60°C at pH 5.3 for 30 minutes
Used for DNA samples pretreatment to detect 8-OHdG

Nuclease P1 hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat-denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity.

One unit of this enzyme will liberate.

1.0 μmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3'-Nucleotidase and will hydrolyze.

4.0 μmol of orthophosphate from 3'-AMP per min at pH 7.2 at 37°C. This enzyme has an optimal temperature of approximately 70°C, but for a long incubation, a temperature below 60°C is more suitable. It is stable in the pH range of 5 to 8.

SPECIFICATIONS

Enzyme Name	Nuclease P1
Conjugation	Unconjugated
Species	Penicillium Citrinum
Molecular Weight	42 - 50 kDa
Form	Lyophilized
Storage Conditions	+4 °C
Shipping Conditions	–20 °C, blue Ice
Enzyme Activity	3′-5′-Phosphodiesterase: One unit will liberate ~1.0µmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3′-Nucleotidase: One unit will hydrolyze ~4.0µmol of orthophosphate from 3′-AMP per min at pH 7.2 at 37°C.