50

Nuclease P1 from Penicillium citrinum For pretreatment DNA samples to detect 8-OHdG

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SKU:
50-OPNUCP1-GEN
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NULL808.00

Description

Nuclease P1 from Penicillium citrinum For pretreatment DNA samples to detect 8-OHdG

Description

Nuclease P1 is a zinc dependent glycoprotein that catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5'-phosphates and 5'-phospho-oligonucleotides.
  • Consists of 270 amino acid residues
  • Optimal pH: 5.3 (RNA and heat-denatured DNA); 7.2 (3'-AMP)
  • Stable below 60°C at pH 5.3 for 30 minutes
  • Used for DNA samples pretreatment to detect 8-OHdG
Nuclease P1 hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat-denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity.
 
One unit of this enzyme will liberate.
 
1.0 μmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3'-Nucleotidase and will hydrolyze.
 
4.0 μmol of orthophosphate from 3'-AMP per min at pH 7.2 at 37°C. This enzyme has an optimal temperature of approximately 70°C, but for a long incubation, a temperature below 60°C is more suitable. It is stable in the pH range of 5 to 8.

SPECIFICATIONS

Enzyme Name Nuclease P1
Conjugation Unconjugated
Species Penicillium Citrinum
Molecular Weight 42 - 50 kDa
Form Lyophilized
Storage Conditions +4 °C
Shipping Conditions –20 °C, blue Ice
Enzyme Activity 3′-5′-Phosphodiesterase: One unit will liberate ~1.0µmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C.
3′-Nucleotidase: One unit will hydrolyze ~4.0µmol of orthophosphate from 3′-AMP per min at pH 7.2 at 37°C.
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Additional Information

Size:
500 units/vial
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