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Nucleophosmin (hNPM), human recombinant | 7258
- SKU:
- 26-7258-GEN
- Availability:
- Usually shipped in 5 working days
Description
Nucleophosmin is a nucleus protein which belongs to the nucleoplasmin family. It is a nucleolar phosphoprotein that shuttles between the nucleus and cytoplasm during the cell cycle. Nucleophosmin / NPM1 is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53 / p53 and ARF. Subcellular localization of Nucleophosmin / NPM1 appears to be strongly correlated with NPM1 functions and cell proliferation. It is phosphorylated mainly at its central acidic domain by several upstream kinases, and its phosphorylation appears to be involved in regulating its functions in ribosome biogenesis and centrosome duplication. Nucleophosmin / NPM1 binds ribosome presumably to drive ribosome nuclear export. It is associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Nucleophosmin / NPM1 acts as a chaperonin for the core histones H3, H2B and H4. Nucleophosmin / NPM1 may act as a licensing factor to maintain proper centrosome duplication and that the Ran/CRM1 nucleocytoplasmic complex regulates local trafficking of Nucleophosmin / NPM1 to centrosomes by interacting through its nuclear export sequence motif. The recombinant protein includes NPM (292 aa) with N-terminal GST-tag.
7258 | Nucleophosmin hNPM human recombinant DataSheet
Biomolecule/Target: Nucleophosmin
Synonyms: Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NPM, NPM1
Alternates names: Protein L
Taglines: A protein involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly etc.
NCBI Gene ID #: N/A
NCBI Gene Symbol: N/A
Gene Source: Peptostreptococcus Magnus
Accession #: CAA25612
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: <0.1 ng/g
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 43.2 kDa with 5 IgG binding sites
Concentration: N/A
Appearance: Liquid
Physical form description: Lyophilized with no additives
Reconstitution Instructions: Reconstitution in HO to a concentration of 5 mg/ml gives a clear solution.
Amino acid sequence: N/A