Description
NUP85 Antibody | 45-102 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen: The immunogen for this antibody is: C-ARAIIREGSLEGS
Research Area: Homeostasis
Tested Application: E, WB, IP
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120) . Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Toda E et al, J Immunol. 2009 Nov 15;183 (10) :6387-94. PMID: 19841162.Immunoprecipitation: This antibody was successfully used in IP on Human: Toda E et al, J Immunol. 2009 Nov 15;183 (10) :6387-94. PMID: 19841162.
Specificiy: Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2) . This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies) .
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: NUP85, pericentrin 1, PCNT1, PCN, PCNT, frount, nucleoporin Nup75, nucleoporin 85kDa, FLJ12549, Nup75, nucleoporin 85
User Note: N/A
BACKGROUND: N/A