O-Propargyl-puromycin | B2590

O-Propargyl-puromycin (OPP) is cell-permeable alkyne analog of the protein synthesis inhibitor puromycin (Cat. No. 1860) that efficiently incorporates into newly translated proteins in complete methionine-containing media. The resulting truncated C-terminal alkyne labeled proteins can subsequently be detected via Cu(I)-catalyzed click chemistry reaction that links it to a fluorescently tagged azide molecule. Thus, OPP provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy, high-content imaging, or flow cytometry.

O-Propargyl-puromycin (OPP) is cell-permeable alkyne analog of the protein synthesis inhibitor puromycin (Cat. No. 1860) that efficiently incorporates into newly translated proteins in complete methionine-containing media. The resulting truncated C-terminal alkyne labeled proteins can subsequently be detected via Cu(I)-catalyzed click chemistry reaction that links it to a fluorescently tagged azide molecule. Thus, OPP provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy, high-content imaging, or flow cytometry.

B2590 | O-Propargyl-puromycin DataSheet

Alternate Name/Synonyms: OPP, (2S)-2-amino-N-((2S,5R)-5-(6-(dimethylamino)-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)-3-(4-(prop-2-yn-1-yloxy)phenyl)propanamide, OP-puro

Appearance: Off-white solid

Formulation:

CAS Number: 1416561-90-4

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₄H₂₉N₇O₅

Molecular Weight: 495.53

Cell-Permeable?: True

Purity: ≥95% by HPLC

Solubilities: >20 mg/ml in DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A nascent protein marker suitable for imaging

MDL Number:

PubChem CID: 71576433

SMILES: CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OCC#C)N)O

InChi: InChI=1S/C24H29N7O5/c1-4-9-35-15-7-5-14(6-8-15)10-16(25)23(34)29-18-17(11-32)36-24(20(18)33)31-13-28-19-21(30(2)3)26-12-27-22(19)31/h1,5-8,12-13,16-18,20,24,32-33H,9-11,25H2,2-3H3,(H,29,34)/t16-,17+,18+,20+,24+/m0/s1

InChi Key: