ORAI1 Antibody | 4041

ORAI1 Antibody | 4041 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: ORAI1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human ORAI1.
The immunogen is located within the first 50 amino acids of ORAI1.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Positive Control 1: Cat. No. 1316 - Human Ovary Tissue Lysate

Positive Control 2: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 3: Cat. No. 11-201 - Human Ovary Tissue Slide

Positive Control 4: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 33 kDa
Observed: 50 kDa

Validation: N/A

Isoform: N/A

Purification: ORAI1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ORAI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: ORAI1 Antibody: IMD9, ORAT1, CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ORAI1 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.