ORAI1 Antibody [6D11A11] | PM-5207

ORAI1 Antibody [6D11A11] | PM-5207 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: ORAI1 antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Positive Control 1: Cat. No. 1366 - Human Spleen Tissue Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 33 kDa
Observed: 50 kDa

Validation: N/A

Isoform: N/A

Purification: ORAI1 Monoclonal Antibody is Protein A purified.

Clonality: Monoclonal

Clone: 6D11A11

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ORAI1 monoclonal antibody can be stored at -20˚C, stable for one year.

Alternate Name: ORAI1 Antibody [6D11A11] : IMD9, ORAT1, CRACM1, TMEM142A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.