ORC1 Antibody | 15-710

ORC1 Antibody | 15-710 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 696-856 of human ORC1 (NP_001177748.1) .

Research Area: Cell Cycle

Tested Application: WB

Application: WB: 1:500 - 1:2000

Specificiy: N/A

Positive Control 1: HeLa

Positive Control 2: 293T

Positive Control 3: A-431

Positive Control 4: K-562

Positive Control 5: Jurkat

Positive Control 6: MCF7

Molecular Weight: Observed: 90kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: HSORC1L, PARC1, ORC1, origin recognition complex subunit 1, origin recognition complex, subunit 1 homolog, replication control protein 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1) , a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.