Osteopontin, human recombinant | 7335

Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

7335 | Osteopontin human recombinant DataSheet

Biomolecule/Target: Osteopontin

Synonyms: OPN, SPP-1, BNSP, BSPI, ETA-1

Alternates names: OPN, SPP-1, BNSP, BSPI, ETA-1

Taglines: Plays an important role in bone remodeling

NCBI Gene ID #: 117

NCBI Gene Symbol: AGER

Gene Source: Human

Accession #: Q15109

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 microgram of protein

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 36.2 kDa (321 aa, 17-314 aa + His Tag), confirmed by MALDI-TOF. (MW on SDS-PAGE shifts up)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, and 2 mM EDTA.

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml.). After adding 1x PBS, let the tube stand at room temperature for 3 minutes to allow lyophilized protein to dissolve. Mix the solution by inverting the tube 5 times. Centrifuge to pool sample.

Amino acid sequence: Recombinant human AGER/RAGE produced by transfected human cell is a secreted protein with sequence (Ala23-Ala344) of human AGER/RAGE (Uniprot Entry: Q15109) fused with a poly-histidine tag at the C-terminus.