OSTF1, human recombinant | 7336

Osteoclast-stimulating factor-1 (OSTF1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast (OCL) formation and bone resorption. OSTF1 is highly expressed in OCL and contains structural domains such as SH3 and ankyrin repeats, which are potentially involved in protein-protein interactions. OSTF1 is ubiquitously expressed analogous to other regulators of OCL activity such as c-Src or transforming growth factor beta. Recombinant human OSTF1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7336 | OSTF1 human recombinant DataSheet

Biomolecule/Target: OSTF1

Synonyms: Osteoclast stimulating factor 1, SH3P2, OSF

Alternates names: Osteoclast stimulating factor 1, SH3P2, OSF

Taglines: Indirectly induces osteoclast (OCL) formation and bone resorption

NCBI Gene ID #: 117

NCBI Gene Symbol: AGER

Gene Source: Human

Accession #: Q15109

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 microgram of protein

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25.1 kDa (225 aa, 1-217 aa + His Tag), confirmed by MALDI-TOF. (MW on SDS-PAGE shifts up)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 0.5 mM DTT.

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml.). After adding 1x PBS, let the tube stand at room temperature for 3 minutes to allow lyophilized protein to dissolve. Mix the solution by inverting the tube 5 times. Centrifuge to pool sample.

Amino acid sequence: Recombinant human AGER/RAGE produced by transfected human cell is a secreted protein with sequence (Ala23-Ala344) of human AGER/RAGE (Uniprot Entry: Q15109) fused with a poly-histidine tag at the C-terminus.