OTU7B Antibody (C-term) [APR03939G]

OTU7B Antibody (C-term) [APR03939G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This OTU7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-784 amino acids from the C-terminal region of human OTU7B.

Cellular Localisation: Cytoplasm. Nucleus Note=Shuttles be cytoplasm and the nucleus in a XPO1/CRM1-dependent manner.

Molecular Weight: 92526

Clone: Polyclonal

Gene Name: OTUD7B

Gene ID: 56957

Function: Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF- kappa-B. Negatively regulates mucosal immunity against infections (By similarity) . Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241) . Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity) . Mediates deubiquitination of EGFR (PubMed:22179831) . Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584) . Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro) ; however the physiological significance of these data are unsure (PubMed:27732584) . Hydrolyzes both linear and branched forms of polyubiquitin.

Summary: Tissue Location: Widely expressed. Abundant in kidney, heart and fetal liver. Expressed differentially among B-cells at distinct developmental stages. Higher expression seen in primary immature B- cells as compared to the mature cells.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: OTU domain-containing protein 7B, Cellular zinc finger anti-NF-kappa-B protein, Zinc finger A20 domain-containing protein 1, Zinc finger protein Cezanne, OTUD7B, ZA20D1