Description
OTUD4 Antibody | 5075 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4.
The immunogen is located within the last 50 amino acids of OTUD4.
Research Area: Cancer
Tested Application: E, WB, ICC, IF
Application: OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: Multiple isoforms of OTUD4 are known to exist. OTUD4 antibody is predicted to not cross-react with other members of the OTUD family.
Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 115, 123 kDa
Observed: 125 kDa
Validation: N/A
Isoform: N/A
Purification: OTUD4 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: OTUD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: OTUD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: OTUD4 Antibody: HIN1, DUBA6, HSHIN1, HIN1, KIAA1046, OTU domain-containing protein 4, HIV-1-induced protein HIN-1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq) .