P-Tyr | Phosphotyrosine (clone G104) | AS10 705

P-Tyr | Phosphotyrosine (clone G104) | AS10 705 | Gentaur UK, US & Europe Distribution

Immunogen: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3- (3'-dimentrylaminopropyl) carbodiimide

Host: Mouse

Conjugation: N/A

Clonality: Monoclonal

Isotype: IgG1

Purity: Total IgG.

Format: Liquid

Tested Application: Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Related Products: Plant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: N/A

Confirmed Reactivity: Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated, Does not cross react with phosphoserine or phosphothreonine

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: 1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis

Background: Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.

Reconstitution: N/A

Storage: Store at -20°C for one year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Signal transduction

Research Area: Cell Signaling