P2RX7 Antibody | 8015

P2RX7 Antibody | 8015 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (81%)

Immunogen: P2RX7 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human P2RX7.
The immunogen is located within the last 50 amino acids of P2RX7.

Research Area: Signal Transduction, Immunology

Tested Application: E, WB, ICC, IF

Application: P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist.

Positive Control 1: Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 2: Cat. No. 17-201 - 3T3/BALB Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 34, 47, 65 kDa
Observed: 54 kDa

Validation: N/A

Isoform: N/A

Purification: P2RX7 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: P2RX7 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: P2RX7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: P2X purinoceptor 7, P2X7, purinergic receptor P2X ligand-gated ion channel 7

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1) . This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1, 2) . Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2) . Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4) .