p38alpha, Active | 7756

p38a (SAPK2A) is a member of the p38 MAPK family which are activated by various environmental stresses and proinflammatory cytokines (1). The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase (2). The substrates of p38 include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response (5).

7756 | p38alpha Active DataSheet

Biomolecule/Target: N/A

Synonyms: p38, mitogen-activated protein kinase 14

Alternates names: p38, mitogen-activated protein kinase 14

Taglines: Involved in a wide variety of cellular processes

NCBI Gene ID #: 117

NCBI Gene Symbol: AGER

Gene Source: N/A

Accession #: Q15109

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of p38alpha was determined to be 77 nmol /min/mg as per activity assay protocol.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 67.0 kDa

Concentration: 0.1mg/ml

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml.). After adding 1x PBS, let the tube stand at room temperature for 3 minutes to allow lyophilized protein to dissolve. Mix the solution by inverting the tube 5 times. Centrifuge to pool sample.

Amino acid sequence: Recombinant human AGER/RAGE produced by transfected human cell is a secreted protein with sequence (Ala23-Ala344) of human AGER/RAGE (Uniprot Entry: Q15109) fused with a poly-histidine tag at the C-terminus.