P4HA1 Antibody | 46-124

P4HA1 Antibody | 46-124 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog

Immunogen: The immunogen for this antibody is: C-DFARKDEPDAFKE

Research Area: Cancer

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1) . Recommended concentration: 0.1-0.1ug/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Spleen. Recommended concentration: 5ug/ml.

Specificiy: This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1) .

Positive Control 1: Cat. No. 1210 - 293 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: P4HA1, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase) , alpha polypeptide I, 4-PH alpha-1, P4HA, prolyl 4-hydroxylase, alpha I subunit

User Note: N/A

BACKGROUND: N/A