PABPC1 Antibody (Center) [APR32028G]

PABPC1 Antibody (Center) [APR32028G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This PABPC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human PABPC1.

Cellular Localisation: Cytoplasm. Cytoplasm, Stress granule. Nucleus. Cell projection, lamellipodium. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:17289661) . Shuttles between the cytoplasm and the nucleus (PubMed:9582337) . During stress and in the absence of DDX3X, localizes to the nucleus (PubMed:21883093) . At the leading edge of migrating fibroblasts, colocalizes with DDX3X (PubMed:28733330) . Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with ENDOV (PubMed:27573237) . In case of HRSV infection, localizes in cytoplasmic inclusion bodies substructures called inclusion bodies associated granules (IBAGs) (PubMed:31649314) .

Molecular Weight: 70671

Clone: Polyclonal

Gene Name: PABPC1 (HGNC:8554)

Gene ID: 26986

Function: Binds the poly (A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299) . Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744) . Can probably bind to cytoplasmic RNA sequences other than poly (A) in vivo. Involved in translationally coupled mRNA turnover (PubMed:11051545) . Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545) . Involved in regulation of nonsense- mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome- bound release factors is proposed (PubMed:18447585) . By binding to long poly (A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299) .

Summary: Tissue Location: Ubiquitous.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Polyadenylate-binding protein 1, PABP-1, Poly (A) -binding protein 1, PABPC1, PAB1, PABP1, PABPC2