PACS1 Antibody | 8187

PACS1 Antibody | 8187 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: PACS1 antibody was raised against a 17 amino acid peptide near the center of human PACS1.
The immunogen is located within amino acids 430 - 480 of PACS1.

Research Area: Homeostasis, Infectious Disease, Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: PACS1 antibody can be used for detection of PACS1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: PACS1 antibody is human, mouse and rat reactive. Multiple isoforms of PACS1 are known to exist. PACS1 antibody is predicted to not cross-react with PACS2.

Positive Control 1: Cat. No. 1478 - Rat Bladder Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 106 kDa
Observed: 105 kDa

Validation: N/A

Isoform: N/A

Purification: PACS1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PACS1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PACS1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Phosphofurin acidic cluster sorting protein 1, PACS-1, MRD17

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The phosphofurin acidic cluster sorting protein 1 (PACS1) , belongs to the PACS family of cytosolic connector proteins that localize different membrane proteins to the trans-Golgi network (TGN) (1, 2) . Mutations in PACS1 cause defects in cranial-neural crest migration during early development, leading to a recognizable intellectual disability syndrome (3) . PACS1, along with the related protein PACS2, also plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance (4) .