PACSIN1 Antibody (Center) [APR17730G]

PACSIN1 Antibody (Center) [APR17730G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This PACSIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 amino acids from the Central region of human PACSIN1.

Cellular Localisation: Cytoplasm. Cell projection. Cell junction, synapse, synaptosome. Cell projection, ruffle membrane. Membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Cell junction, synapse. Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with MAPT in axons. In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites (By similarity) . Associates with membranes via its F-BAR domain.

Molecular Weight: 50966

Clone: Polyclonal

Gene Name: PACSIN1

Gene ID: 29993

Function: Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity) . Binds to membranes via its F-BAR domain and mediates membrane tubulation.

Summary: Tissue Location: Highly expressed in brain and, at much lower levels, in heart and pancreas.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, WB

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Protein kinase C and casein kinase substrate in neurons protein 1, Syndapin-1, PACSIN1, KIAA1379