PAK1/2 (P429) polyclonal Antibody | BS5530

PAK1/2 (P429) polyclonal Antibody | BS5530 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PAK1/2 (P429) pAb detects endogenous levels of PAK1 and PAK2 protein.

Molecular Weight: ~ 60 to 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Serine/threonine-protein kinase PAK 1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK; PAK1; PAKα; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; PAK-2p27; PAK-2p34; C-t-PAK2; PAK2; PAKγ

Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human PAK1.

Conjugate: N/A

Modification: N/A

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: N/A