Description
PAK4 is a recently identified member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, cdc 42 and Rac. PAK4 belongs to the Group II PAKs which also includes PAK5 and PAK6 while Group I PAKs comprise of PAK1, PAK2 and PAK3. PAK4 differs from other members of the PAK family both in sequence and function. PAK4 has been shown to regulate cell morphology and cytoskeletal organization in mammalian cells. PAK4 regulates the activity of LIM kinase 1 which in turn phosphorylates Cofilin leading to cytoskeletal changes. PAK4 can protect cells from apoptosis in response to several different types of stimuli by inhibiting the pro-apoptotic proteins Bad and Caspase 8. PAK4 has been shown to associate with and mediate the downstream signaling of the keratinocyte growth factor receptor. In addition, PAK4 interacts with the 5 integrins and regulates cell migration during engagement of the v5 integrin receptor.
Biomolecule/Target: N/A
Synonyms: PAK, Serine/threonine-protein kinase
Alternates names: PAK, Serine/threonine-protein kinase
Taglines: Target for the small GTP binding proteins
NCBI Gene ID #: 627
NCBI Gene Symbol: BDNF
Gene Source: N/A
Accession #: P23560
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): 83 nmol phosphate incorporated into the PKB-sub peptide (CKRPRAASFAE) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: ~90.0 kDa
Concentration: 5µg/50 µl
Appearance: Liquid
Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100g/ml.). This can further be diluted to other aqueous buffers.
Amino acid sequence: Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.