PAK7, Active | 7727

The p21-activated kinase (PAK) family of protein kinases has recently attracted considerable attention as an effector of Rho family of small G proteins and as an upstream regulator of MAPK signalling pathways during cellular events such as re-arrangement of the cytoskeleton and apoptosis. PAK7 is a novel human PAK family kinase that contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK7 like the other Paks has been implicated in the regulation of cell morphology, motility and transformation.

7727 | PAK7 Active DataSheet

Biomolecule/Target: N/A

Synonyms: PAK7, Serine/threonine-protein kinase PAK 7

Alternates names: PAK7, Serine/threonine-protein kinase PAK 7

Taglines: Target for the small GTP binding proteins

NCBI Gene ID #: 627

NCBI Gene Symbol: BDNF

Gene Source: N/A

Accession #: P23560

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 55 nmol phosphate incorporated into the Akt substrate peptide (CKRPRAASFAE) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 130.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100g/ml.). This can further be diluted to other aqueous buffers.

Amino acid sequence: Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.