Parg Antibody (C-term) [APR32409G]

Parg Antibody (C-term) [APR32409G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This Parg antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-421 amino acids from the C-terminal region of human Parg.

Cellular Localisation: [Isoform 1]: Nucleus Note=Colocalizes with PCNA at replication foci (PubMed:21398629) Relocalizes to the cytoplasm in response to DNA damage (PubMed:16460818) . [Isoform 3]: Cytoplasm [Isoform 5]: Mitochondrion matrix

Molecular Weight: 111110

Clone: Polyclonal

Gene Name: PARG {ECO:0000303|PubMed:14527731, ECO:0000312|HGNC:HGNC:8605}

Gene ID: 670;8505

Function: Poly (ADP-ribose) glycohydrolase that degrades poly (ADP- ribose) by hydrolyzing the ribose-ribose bonds present in poly (ADP- ribose) (PubMed:15450800, PubMed:21892188, PubMed:23102699, PubMed:23474714, PubMed:33186521) . PARG acts both as an endo- and exoglycosidase, releasing poly (ADP-ribose) of different length as well as ADP-ribose monomers (PubMed:23102699, PubMed:23481255) . It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP- ribosylated (PubMed:21892188, PubMed:23474714, PubMed:33186521) . Poly (ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG (PubMed:23102699) . Required to prevent detrimental accumulation of poly (ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed:24906880) . Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly (ADP-ribose) without cleaving the terminal protein-ribose bond (PubMed:33186521) . Required for retinoid acid- dependent gene transactivation, probably by removing poly (ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR- dependent gene promoters (PubMed:23102699) . Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257) . Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257) .

Summary: Tissue Location: Ubiquitously expressed.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, WB

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Poly (ADP-ribose) glycohydrolase, PARG