PARP2 Antibody (C-term) [APR30933G]

PARP2 Antibody (C-term) [APR30933G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This PARP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-336 amino acids from the C-terminal region of human PARP2.

Cellular Localisation: Nucleus. Chromosome {ECO:0000250|UniProtKB:Q9UGN5}. Note=Recruited to DNA damage sites {ECO:0000250|UniProtKB:Q9UGN5}

Molecular Weight: 63397

Clone: Polyclonal

Gene Name: Parp2

Gene ID: 11546

Function: Poly-ADP-ribosyltransferase that mediates poly-ADP- ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231) . Mediates glutamate, aspartate or serine ADP- ribosylation of proteins: the ADP-D-ribosyl group of NAD (+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (By similarity) . Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (By similarity) . Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (By similarity) . Following interaction with HPF1, catalyzes serine ADP- ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (By similarity) . PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (By similarity) . In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'- terminal phosphates at DNA strand breaks termini in nicked duplex (By similarity) .

Summary: Tissue Location: Widely expressed; the highest levels were in testis followed by ovary (PubMed:11133988) . Expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult (PubMed:11948190) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Poly [ADP-ribose] polymerase 2, PARP-2, mPARP-2, ADP-ribosyltransferase diphtheria toxin-like 2, ARTD2, NAD (+) ADP-ribosyltransferase 2, ADPRT-2, Poly[ADP-ribose] synthase 2, pADPRT-2, Parp2, Adprt2, Adprtl2, Aspartl2