PC | Plastocyanin | AS06 141

PC | Plastocyanin | AS06 141 | Gentaur UK, US & Europe Distribution

Immunogen: Purified native plastocyanin from Spinacia oleracea UniProt: P00289

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunogold (IG), Western blot (WB)

Related Products: AS06 202 | Anti-cytc6 marker antibody of thylakoid lumen for Chlamydomonas reinhardtii, rabbit antibodiesPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 100 (IG), 1 : 2000 (WB)

Molecular weight: 10 kDa

Confirmed Reactivity: Arabidops thaliana, Brassica juncea, Heliantus annuus, Hordeum vulgare, Lathyrus sativus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Synechocystis sp. PCC6803, Zea mays

Predicted Reactivity: Catalpa bungei, Dicots, Chlamydomonas reinhardtii, Nicotiana benthamiana, Physcomitrium patens, Ricinus communis, Solanum lycopersicumSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.

Background: Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G20340

Category: Electron transfer

Research Area: Plant Biology, Photosynthesis