PC4 Antibody | 31-238

PC4 Antibody | 31-238 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Zebrafish

Homology: N/A

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PC4.

Research Area: Transcription

Tested Application: E, WB, IHC

Application: PC4 antibody can be used for detection of PC4 by ELISA at 1:7812500. PC4 antibody can be used for detection of PC4 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

Specificiy: N/A

Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 14 kDa

Validation: N/A

Isoform: N/A

Purification: Antibody is supplied as total IgG.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Concentration: batch dependent

Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store PC4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Alternate Name: PC4, P15, PC4, p14

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.