PCNA Antibody | 43-158

PCNA Antibody | 43-158 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Rat, Pig

Homology: Expected Species Reactivity based on sequence homology: Dog, Cow

Immunogen: The immunogen for this antibody is: C-NGNIKLSQTSNVD

Research Area: Cancer, Transcription

Tested Application: E, WB, EIA

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to Human NP_002583.1) . Approx.33kDa band observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis and Pig Spleen lysates (calculated MW of 28.8kDa according to Mouse NP_035175.1, Rat NP_071776.1 and Pig NP_001278854.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al,, J Cell Biol. 2009 Sep 7;186 (5) :645-54.) . Recommended concentration: 0.01-0.03ug/ml.Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB11651.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificiy: Reported variants represent identical protein: NP_872590.1, NP_002583.1

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Approx 30kDa

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin

User Note: N/A

BACKGROUND: N/A