PD1 Antibody [10B3] | RF16005

PD1 Antibody [10B3] | RF16005 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: PD-1 antibody was raised against the extracellular domain of human PD-1.

Research Area: Immunology

Tested Application: E, WB, IHC-P, ICC, IF, Flow

Application: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Flow cytometry at 0.1 μg/ml.
Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 2: Cat. No. 1315 - Human Tonsil Tissue Lysate

Positive Control 3: Cat. No. 1369 - Human Lymph Node Tissue Lysate

Positive Control 4: Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 32 kDa
Observed: 38 kDa

Validation: N/A

Isoform: N/A

Purification: PD-1 Antibody is supplied as protein A purified IgG1.

Clonality: Monoclonal

Clone: 10B3

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/mL

Storage Condition: PD-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.