PDCL3 Antibody | 7889

PDCL3 Antibody | 7889 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: PDCL3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human PDCL3.
The immunogen is located within the last 50 amino acids of PDCL3.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 26 kDa
Observed: 25 kDa

Validation: N/A

Isoform: N/A

Purification: PDCL3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PDCL3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PDCL3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: PDCL3 Antibody: VIAF, PHLP3, VIAF1, HTPHLP, PHLP2A, PhLP2A, Phosducin-like protein 3, VIAF-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1, 2) . PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3) . Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4) . Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5) .