PDGF-BB, human recombinant | 4488

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-BB is a 24.3 kDa B chain homodimer protein formed by subunits of 109 amino acid residues.

4488 | PDGF-BB human recombinant DataSheet

Biomolecule/Target: PDGF-BB

Synonyms: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor polypeptide, Becaplermin.

Alternates names: soluble IL-2 receptor, TAC-antigen, CD25 antigen

Taglines: A growth factor that regulates cell growth and division

NCBI Gene ID #: 3561

NCBI Gene Symbol: IL2RA

Gene Source: Human

Accession #: P31785

Recombinant: Yes

Source: (BTI-Tn-5B1-4) Hi-5 Insect Cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to increase the proliferation effect of IL-2 in mouse CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5 g/ml.

Biological activity: Determined by its ability to increase the proliferation effect of IL-2 in mouse CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 24.8 kDa (31 kDa on SDSPAGE due to glycosylation)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in 1x PBS pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ