PDGFR beta, Active | 7770

PDGFRb (platelet-derived growth factor receptor b) is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity. PDGFRb deficient mice are hemorrhagic, severely anemic and exhibit a defect in kidney glomeruli function (1). However, absence of PDGFRb has no impact on major blood vessels and the heart. PDGFRb expression and activity is elevated in several cancers and inhibition of PDGFRb activity blocks progression of renal carcinoma in an animal model (2).

7770 | PDGFR beta Active DataSheet

Biomolecule/Target: N/A

Synonyms: PDGR. platelet-derived growth factor receptor, beta polypeptide

Alternates names: PDGR. platelet-derived growth factor receptor, beta polypeptide

Taglines: Important factors regulating cell proliferation, cellular differentiation, cell growth and development

NCBI Gene ID #: 6358

NCBI Gene Symbol: CCL14

Gene Source: N/A

Accession #: Q16627

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of PDGFRb was determined to be 20 nmol /min/mg as per activity assay protocol.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 96.0 kDa

Concentration: 0.1mg/ml

Appearance: Liquid

Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml.). This can further be diluted to other aqueous buffers.

Amino acid sequence: Target protein is expressed with sequence (Thr20-Asn93) of Human hcc-1 (Uniprot #Q16627) fused with a polyhistidine tag at the C-terminus.