PDK, Active, M. rosea Recombinant | P1161

Pyruvate phosphate dikinase, PPDK, (E.C. 2.7.9.1) is found in certain microorganisms and plants. PPDK catalyzes the interconversion of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi, and pyruvate monophosphate. PPDK plays an important role in the glycolytic pathway and in organisms where pyruvate kinase (PK) is absent. PPDK functions in the process of ATP synthesis. ’s active PPDK is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.

P1161 | PDK Active M. rosea Recombinant DataSheet

Biomolecule/Target: PPDK

Synonyms: Pyruvate Phosphate Dikinase, Pyruvate, orthophosphate dikinase

Alternates names: Pyruvate Phosphate Dikinase, Pyruvate, orthophosphate dikinase

Taglines: Plays an important role in the glycolytic pathway

NCBI Gene ID #: 6358

NCBI Gene Symbol: CCL14

Gene Source: Microbispora rosea

Accession #: Q16627

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by the conversion of 1 mole of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate at 37ºC and pH 7.0.

Biological activity: N/A

Results: 0.8 U/mg

Binding Capacity: N/A

Unit Definition: One unit of enzyme converts 1 mole of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate at 37ºC and pH 7.0.

Molecular Weight: 95.5 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Proprietary buffer

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml.). This can further be diluted to other aqueous buffers.

Amino acid sequence: Target protein is expressed with sequence (Thr20-Asn93) of Human hcc-1 (Uniprot #Q16627) fused with a polyhistidine tag at the C-terminus.