Pellino 1 Antibody | 7071

Pellino 1 Antibody | 7071 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Rabbit polyclonal Pellino 1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Pellino 1.
The immunogen is located within amino acids 300 - 350 of Pellino 1.

Research Area: Innate Immunity

Tested Application: E, WB, ICC, IF

Application: Pellino 1 antibody can be used for detection of Pellino 1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate

Positive Control 2: Cat. No. 17-011 - Hep G2 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 46 kDa

Validation: N/A

Isoform: N/A

Purification: Pellino 1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Pellino 1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Pellino 1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Pellino 1 Antibody: PRISM, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-related intracellular-signaling molecule, Pellino-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Pellino 1 Antibody: The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-κB. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-ε and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.