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Pentraxin 3, human recombinant | 7307
- SKU:
- 26-7307-GEN
- Availability:
- Usually shipped in 5 working days
Description
Pentraxin 3 (PTX3) is the prototypic member of the long pentraxin family sharing the C-terminal domain with short pentraxins and containing a unique N-terminal domain. Pentraxin 3 is produced and released at inflammatory sites by diverse cell types including monocytes/macrophages, endothelial cells, vascular smooth muscle cells, fibroblasts, and adipocytes. It plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Recombinant human Pentraxin 3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
7307 | Pentraxin 3 human recombinant DataSheet
Biomolecule/Target: PTX3
Synonyms: TNFAIP5, TSG-14, PTX3.
Alternates names: TNFAIP5, TSG-14, PTX3.
Taglines: Plays a role in the regulation of innate resistance to pathogens
NCBI Gene ID #: 3627
NCBI Gene Symbol: CXCL10
Gene Source: Human
Accession #: P02778
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 microgram of protein
Activity (Specifications/test method): N/A
Biological activity: The ED50 of rHu CXCL10 as determined by its ability to chemoattract human CXCR3 transfected BaF3 mouse proB cells was 0.02-0.06 g/mL.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 44.4 kDa (401 aa, 18-381 aa + His Tag), confirmed by MALDI-TOF.
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 1 mM DTT.
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml). This can further be diluted to other aqueous buffers.
Amino acid sequence: Recombinant human CXCL10/IP-10/CRG-2 is produced by E.coli transformed with a plasmid contains sequence (Val22-Pro98) of human CXCL10/IP-10/CRG-2 (Uniprot Entry: P02778) fused with a polyhistidine tag at the C-terminus. The sequence of the first five N-terminal amino acids was determined and was found to be Val-Pro-Leu-Ser-Arg.