Peptostreptococcus magnus Protein L | IMS02-136-331

Peptostreptococcus magnus Protein L | IMS02-136-331 | Gentaur UK, US & Europe Distribution

Immunogen: Purified, full length recombinant protein

Host: Chicken

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified IgY in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide., pH 7.2.

Format: Liquid

Tested Application: Affinity purified IgY

Related Products: IMS02-008-333 | Anti-Staphylococcal Protein A, chicken antibodiesIMS02-109-334 | Anti-Streptococcal Protein G, chicken antibodiesSecondary antibodies

Recommended Dilutions: 1 : 5 000 (WB)

Molecular weight: 36 kDa

Confirmed Reactivity: Peptostreptococcus magnus

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: Protein L is a 36 kDa immunoglobulin-binding protein isolated from the bacteria Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L. Protein L binding is restricted to those antibodies that contain kappa light chains and it is only effective in binding certain subtypes of kappa light chains. Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLkappa-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.

Reconstitution: N/A

Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Bacteria

Research Area: Pathology, Peptostreptococcus