PERP Antibody | 2451

PERP Antibody | 2451 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat (100%) ; Mouse (82%)

Immunogen: Anti-PERP antibody (2451) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human PERP.
The immunogen is located within the last 50 amino acids of PERP.

Research Area: Apoptosis

Tested Application: E, WB, ICC

Application: WB: 1-2 μg/mL; ICC: 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 21kD
Observed: 21 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar PERP expression profile in human cell lines detected by two independent anti-PERP antibodies that recognize different epitopes, 2451 against C-terminus domain and 57-777 against C-terminus domain.  PERP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human PERP has one isoform (193aa, 21kD) . Mouse PERP has one isoform (193aa, 21.6kD) and Rat PERP also has one isoform (193aa, 21.6kD) . 2451 can detect human, mouse and rat.

Purification: PERP Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PERP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PERP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PERP Antibody: THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik, Krtcap1, p53 apoptosis effector related to PMP-22, Keratinocyte-associated protein 1, KCP-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PERP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.