PGK1, human recombinant | 7819

Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

7819 | PGK1 human recombinant DataSheet

Biomolecule/Target: PGK1

Synonyms: Phosphoglycerate kinase 1, MGC117307, MGC142128, MGC8947, MIG10, PGKA

Alternates names: Phosphoglycerate kinase 1, MGC117307, MGC142128, MGC8947, MIG10, PGKA

Taglines: Plays a key role in the glycolytic pathway

NCBI Gene ID #: 9547

NCBI Gene Symbol: CXCL14

Gene Source: Human

Accession #: O95715

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED50 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.

Results: Specific activity is >190 units/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 46.8 kDa (437 aa, 1-417 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 1 mM DTT

Reconstitution Instructions: Reconstitute the lyophilized recombinant human CXCL14 in sterile 18M-cm H2O not less than 100 g/ml. This can further be diluted to other aqueous buffers.

Amino acid sequence: Recombinant Human CXCL14 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 9.4 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys.