PHC3 Antibody | 7607

PHC3 Antibody | 7607 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: PHC3 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHC3.
The immunogen is located within amino acids 240 - 290 of PHC3.

Research Area: Stem Cell

Tested Application: E, WB

Application: PHC3 antibody can be used for detection of PHC3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Specificiy: PHC3 antibody is human, mouse and rat specific. At least six isoforms of PHC3 are known to exist.

Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 109 kDa
Observed: 120 kDa

Validation: N/A

Isoform: N/A

Purification: PHC3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PHC3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PHC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: PHC3 Antibody: EDR3, HPH3, EDR3, PH3, Polyhomeotic-like protein 3, Early development regulatory protein 3, hPH3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development (1) . PHC3 (Polyhomeotic-like protein 3) , also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex (2) . PRC1 complex acts via chromatin remodeling and modification of histones. PHC3 contains 1 FCS-type zinc finger and SAM (sterile alpha motif) domain (2-3) . PHC 3 has been regarded as a candidate tumor suppressor of osteosarcoma (4) .