Phospho Histone H2AX (Ser139) Antibody | A1957

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

A1957 | Phospho Histone H2AX (Ser139) Antibody DataSheet

Antibody Target: Phospho Histone H2AX (Ser139)

Target Alternative Name: H2AX; Histone H2AX; H2a/x; Histone H2A.X

Tag Line: Affinity purified rabbit polyclonal antibody to detect phosphorylation of Histone H2AX at Ser139 in human, mouse and rabbit samples

Category: Primary antibody

Host: Rabbit

Isotype: IgG

Species Reactivities: Rabbit, Mouse, Human

Immunogen Sequence: KLH-conjugated synthetic peptide targeting a sequence within the C-term region of human Histone H2AX

Accession #: P16104

Gene ID: 3014

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In 0.42% Potassium phosphate; 0.87% NaCl; pH 7.3; 30% glycerol; and 0.01% sodium azide

Purification: Affinity purified

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting, Flow Cytometry

Positive Control:

Application And Usages: WB 1:500 - 1:1000, IHC 1:100 - 1:200, IP 1:10 - 1:100, FC 1:100 - 1:200

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: True

Immunocytochemistry Verified: True

Immunofluorescence Verified: False

Immunoprecipitation Verified: True

FACS Verified: False

ELISA Verified: False

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: True