Phospho-SPRY4 (Y75) Antibody [AMM07158G]

Phospho-SPRY4 (Y75) Antibody [AMM07158G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This SPRY4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y75 of human SPRY4.

Cellular Localisation: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor) (By similarity) . Colocalizes with TESK1 in vesicular spots in the cytoplasm (PubMed:15584898) {ECO:0000250|UniProtKB:Q9WTP2, ECO:0000269|PubMed:15584898}

Molecular Weight: 32541

Clone: Polyclonal

Gene Name: SPRY4

Gene ID: 81848

Function: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed:12027893) . Probably impairs the formation of GTP-Ras (PubMed:12027893) . Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed:12717443) . Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:15584898) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, DB

Dilution: WB--1:1000 DB--1:500

Synonyms: Protein sprouty homolog 4, Spry-4, SPRY4