Phosphotyrosine Antibody [PY20] (HRP) | 98-516

Phosphotyrosine Antibody [PY20] (HRP) | 98-516 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: N/A

Homology: N/A

Immunogen: Phosphotyrosine conjugated to carrier proteins

Research Area: Obesity, Signal Transduction, Neuroscience, Cancer

Tested Application: E

Application: Phosphotyrosine Antibody [PY20] for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.

Specificiy: Phosphotyrosine

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [PY20]

Isotype: IgG2b

Conjugate: HRP

Physical State: Liquid

Buffer: Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added

Concentration: Lot specific

Storage Condition: Store vial at 2-8˚C

Alternate Name: pTyr20

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate.