PIAS2 (PIASx1/2) Antibody (C-term) [APR30962G]

PIAS2 (PIASx1/2) Antibody (C-term) [APR30962G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This PIAS2 (PIASx1/2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 518-547 amino acids from the C-terminal region of human PIAS2 (PIASx1/2) .

Cellular Localisation: Nucleus speckle {ECO:0000250|UniProtKB:Q8C5D8}. Nucleus, PML body. Nucleus. Note=Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621) Colocalizes with SUMO1 in nuclear granules (By similarity) {ECO:0000250|UniProtKB:Q8C5D8, ECO:0000269|PubMed:22406621}

Molecular Weight: 68240

Clone: Polyclonal

Gene Name: PIAS2

Gene ID: 9063

Function: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.

Summary: Tissue Location: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P

Dilution: IHC-P--1:100~500

Synonyms: E3 SUMO-protein ligase PIAS2, 632-, Androgen receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, Msx-interacting zinc finger protein, Miz1, PIAS-NY protein, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2, PIAS2, PIASX